Human UBE2I His Recombinant Protein (RPPB2380)
- SKU:
- RPPB2380
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P63279
- Research Area:
- Enzymes
Description
| Product Name: | Human UBE2I His Recombinant Protein |
| Product Code: | RPPB2380 |
| Size: | 50µg |
| Species: | Human |
| Target: | UBE2I His |
| Synonyms: | SUMO-conjugating enzyme UBC9, EC 6.3.2.-, SUMO-protein ligase, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, Ubiquitin carrier protein I, Ubiquitin carrier protein 9, p18, UBC9, C358B7.1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered white lyophilized powder. |
| Formulation: | Lyophilized from a 0.2?m filtered concentrated (1mg/ml) solution in 1X PBS and 1mM DTT, pH 7.5. |
| Solubility: | It is recommended to reconstitute the lyophilized UBE2I in sterile water not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized UBE2I although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution UBE2I should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MHHHHHHAMGTLNMSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS |
Human Ubquitin Conjugating Enzyme 9 (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumolyation, and may play a role in substrate recognition. Ubc9 can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1 and PML without the requirement of an E3 ligase.
Ubiquitin-Conjugating Enzyme E2I Human Recombinant produced in E.coli is a 19.5 kDa protein containing 171 amino acids.The UBE2I protein contains 6xHis tag and is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Function: E2 ubiquitin-like--protein ligase mediating SUMO/Smt3 attachment to septins and PCNA. Seems to be involved in degradation of S- (CLB5) and M-phase cyclins (CLB2). Ref.4 Ref.5 |
| UniProt Protein Details: | Catalytic activity: ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine. Pathway: Protein modification; protein sumoylation. Subunit structure: Interacts with SIZ1. Ref.5 Ref.6 Subcellular location: Nucleus Ref.7. Miscellaneous: Present with 2600 molecules/cell in log phase SD medium. Ref.8 Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family. |
| UniProt Code: | P63279 |
| NCBI GenInfo Identifier: | |
| NCBI Gene ID: | |
| NCBI Accession: | |
| Molecular Weight: | 17,911 Da |
| NCBI Full Name: | UBC9 |
| UniProt Protein Name: | SUMO-conjugating enzyme UBC9 |
| UniProt Synonym Protein Names: | Ubiquitin carrier protein 9; Ubiquitin-conjugating enzyme E2-18 kDa; Ubiquitin-protein ligase |
| UniProt Gene Name: | UBC9�� |
| UniProt Entry Name: | UBC9_YEAST |
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