Human UBE2B Recombinant Protein (RPPB2366)
- SKU:
- RPPB2366
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P63146
- Research Area:
- Enzymes
Description
| Product Name: | Human UBE2B Recombinant Protein |
| Product Code: | RPPB2366 |
| Size: | 10µg |
| Species: | Human |
| Target: | UBE2B |
| Synonyms: | Ubiquitin-conjugating enzyme E2 B, EC 6.3.2.19, Ubiquitin-protein ligase B, Ubiquitin carrier protein B, HR6B, hHR6B, E2-17 kDa UBC2, HHR6B, RAD6B, E2-17kDa, UBE2B. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered white lyophilized powder. |
| Formulation: | Lyophilized from a 0.2?m filtered concentrated (1 mg/ml) solution in 1X PBS and 1mM DTT, pH 7.5. |
| Solubility: | It is recommended to reconstitute the lyophilized UBE2B in sterile water not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized UBE2B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution UBE2B should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MHHHHHHAMGQLRSMSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS |
This E2 enzyme encodes for the human homolog of the yeast DNA repair gene RAD6, which is induced by DNA damaging agents. UBE2B can conjugate ubiquitin to histone H2A in an E3- independent manner in vitro, and is essential for the multi-ubiquitination and degradation of N-end rule substrates. Additionally, UBE2B may have a role in sepsis-induced muscle protein proteolysis and cancer-induced cachexia.
Ubiquitin Conjugating Enzyme E2B Human Recombinant produced in E.coli is a 19 kDa protein containing 166 amino acids.The UE2B protein contains 6xHis tag and is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | UBE2B: a ubiquitin-protein ligase, a member of the ubiquitin-conjugating enzyme family. Homologous to the yeast DNA repair gene RAD6. Interacts with RAD18. Required for postreplication repair of UV-damaged DNA. |
| UniProt Protein Details: | Protein type:Ligase; EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase Chromosomal Location of Human Ortholog: 5q31.1 Cellular Component: XY body; nuclear chromatin; cytoplasm; plasma membrane; replication fork; chromatin; nucleus Molecular Function:protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity Biological Process: response to drug; protein monoubiquitination; ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein autoubiquitination; protein polyubiquitination; protein stabilization; in utero embryonic development; postreplication repair; protein ubiquitination; Wnt receptor signaling pathway through beta-catenin; DNA repair; negative regulation of histone phosphorylation; cellular response to insulin stimulus; maintenance of chromatin silencing; response to hypoxia; sperm axoneme assembly; spermatogenesis; chiasma formation; response to DNA damage stimulus; histone H2A ubiquitination; response to UV; negative regulation of apoptosis |
| NCBI Summary: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P63146 |
| NCBI GenInfo Identifier: | 52783814 |
| NCBI Gene ID: | 7320 |
| NCBI Accession: | P63146.1 |
| UniProt Related Accession: | P63146 |
| Molecular Weight: | |
| NCBI Full Name: | Ubiquitin-conjugating enzyme E2 B |
| NCBI Synonym Full Names: | ubiquitin conjugating enzyme E2 B |
| NCBI Official Symbol: | UBE2B�� |
| NCBI Official Synonym Symbols: | HR6B; UBC2; HHR6B; RAD6B; E2-17kDa�� |
| NCBI Protein Information: | ubiquitin-conjugating enzyme E2 B |
| UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 B |
| UniProt Synonym Protein Names: | RAD6 homolog B; HR6B; hHR6B; Ubiquitin carrier protein B; Ubiquitin-conjugating enzyme E2-17 kDa; Ubiquitin-protein ligase B |
| Protein Family: | Ubiquitin-conjugating enzyme |
| UniProt Gene Name: | UBE2B�� |
| UniProt Entry Name: | UBE2B_HUMAN |
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