Human UBA52 (Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1) CLIA Kit
Based on the information provided in the URL, here is a product description:The Human UBA52 (Ubiquitin A-52 Residue Ribosomal Protein Fusion Product 1) CLIA Kit is a cutting-edge assay designed for the precise and sensitive detection of UBA52 levels in various biological samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional accuracy and reproducibility, ensuring reliable results for a wide range of research applications.UBA52 is a vital protein involved in the regulation of protein degradation and cellular processes.
It plays a critical role in maintaining cellular homeostasis and function, making it a key biomarker for studying biological pathways and disease mechanisms. The Human UBA52 CLIA Kit facilitates the study of UBA52 levels in various health conditions, offering valuable insights for potential therapeutic interventions.Overall, the Human UBA52 CLIA Kit provides researchers with a powerful tool for investigating UBA52 biology and its implications in human health and disease.
Product Name:
Human UBA52 (Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1) CLIA Kit
SKU:
HUES01222
Target:
Human UBA52 (Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human UBA52. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human UBA52 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UBA52, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human UBA52. You can calculate the concentration of Human UBA52 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-107
101-113
90-103
Average (%)
102
107
96
1:4
Range (%)
96-110
88-98
96-109
Average (%)
103
93
104
1:8
Range (%)
98-114
93-106
88-103
Average (%)
105
99
96
1:16
Range (%)
97-113
92-104
89-99
Average (%)
104
99
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
104
EDTA plasma (n=5)
99-116
106
Cell culture media (n=5)
97-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
206.43
617.17
1877.26
190.28
606.66
2035.73
Standard deviation
24.07
61.41
121.08
20.3
69.64
216.4
C V (%)
11.66
9.95
6.45
10.67
11.48
10.63
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human UBA52 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human UBA52 in samples. No significant cross-reactivity or interference between Human UBA52 and analogues was observed.
