Human Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1) ELISA Kit
The Human Tyrosine Kinase with Immunoglobulin-like and EGF-like Domains Protein-1 (TIE1) ELISA Kit is a highly reliable and sensitive tool for detecting TIE1 levels in human samples, including serum, plasma, and cell culture supernatants. This ELISA kit offers high specificity, ensuring accurate and reproducible results for various research applications.TIE1 is a crucial protein involved in vascular development and endothelial cell function. It plays a significant role in regulating angiogenesis, vascular integrity, and endothelial cell proliferation. Dysregulation of TIE1 expression has been linked to various diseases, including cancer, cardiovascular disorders, and inflammatory conditions, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.
Overall, the Human TIE1 ELISA Kit provides researchers with a valuable tool for investigating the role of TIE1 in vascular biology and related disease pathologies. Its high sensitivity and accuracy make it an essential component for studying the complex mechanisms underlying angiogenesis and vascular development.
Product Name:
Human Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1) ELISA Kit
Product Code:
HUDL02893
Size:
96 Assays
Target:
Tie1
Synonyms:
JTK14, Tyrosine Kinase With Immunoglobulin And Epidermal Growth Factor Homology Domains 1, Tyrosine-protein kinase receptor Tie-1
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
84-100
92
EDTA plasma (n=5)
83-99
91
Heparin plasma (n=5)
85-101
93
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
