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Human Twisted Gastrulation Protein Homolog 1 (TWSG1) ELISA Kit

SKU:
HUDL03022
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9GZX9
Sensitivity:
0.112ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
TSG
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Human Twisted Gastrulation Protein Homolog 1 (TWSG1) ELISA Kit

The Human Twisted Gastrulation Protein Homolog 1 (TWSG1) ELISA Kit is designed for the accurate detection of TWSG1 levels in human serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.TWSG1 is a critical protein involved in regulating bone formation, embryonic development, and cell growth. Dysregulation of TWSG1 has been linked to various diseases, including cancer, skeletal disorders, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.

This ELISA kit offers researchers a powerful tool to accurately quantify TWSG1 levels in biological samples, aiding in the advancement of scientific knowledge and the development of novel treatment strategies. Trust in the Human TWSG1 ELISA Kit for precise and dependable results in your research endeavors.

Product Name:Human Twisted Gastrulation Protein Homolog 1 (TWSG1) ELISA Kit
Product Code:HUDL03022
Size:96 Assays
Target:TWSG1
Synonyms:TSG
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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