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Human Twist Transcription Factor (TWIST) ELISA Kit

SKU:
HUDL03021
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q15672
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ACS3,BPES2,BPES3,SCS,Class A basic helix-loop-helix protein 38,Acrocephalosyndactyly 3,Saethre-Chotzen Syndrome,Blepharophimosis,Epicanthus Inversus And Ptosis 3
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Twist Transcription Factor (TWIST) ELISA Kit

The Human Twist Transcription Factor (TWIST) ELISA Kit is specifically designed for the precise measurement of TWIST levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.TWIST is a key transcription factor that plays a critical role in regulating epithelial-mesenchymal transition (EMT) and cell differentiation processes. Dysregulation of TWIST expression has been linked to various diseases, including cancer, metastasis, and developmental disorders.

As such, measuring TWIST levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its reliable performance and broad applicability, the Human TWIST Transcription Factor ELISA Kit is an essential tool for researchers studying TWIST biology and its implications in health and disease. Discover new insights and advance your research with this cutting-edge ELISA kit from AssayGenie.

Product Name:Human Twist Transcription Factor (TWIST) ELISA Kit
Product Code:HUDL03021
Size:96 Assays
Target:TWIST
Synonyms:ACS3, BPES2, BPES3, SCS, Class A basic helix-loop-helix protein 38, Acrocephalosyndactyly 3, Saethre-Chotzen Syndrome, Blepharophimosis,Epicanthus Inversus And Ptosis 3
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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