Human TWEAK (Tumour Necrosis Factor Related Weak Inducer of Apoptosis) ELISA Kit
The TWEAK ELISA Kit is designed for the quantitative detection of Tumour Necrosis Factor-Related Weak Inducer of Apoptosis (TWEAK) levels in various human biological samples.
TWEAK is a multifunctional cytokine involved in diverse cellular processes, including cell death, proliferation, and differentiation. It plays a significant role in regulating inflammation and apoptosis and has implications in various disease conditions and cellular responses. Accurate measurement of TWEAK is crucial for understanding its functions and its potential role in disease pathology and therapeutic strategies. Our TWEAK ELISA Kit provides exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under rigorous quality control standards, this kit offers robust performance and user-friendly protocols suitable for both research and clinical applications. Trust our TWEAK ELISA Kit for precise and reliable quantification of this essential biomarker in your studies and investigations.
Product Name:
Human TWEAK (Tumour Necrosis Factor Related Weak Inducer of Apoptosis) ELISA Kit
SKU:
AEES00041
Target:
Human TWEAK (Tumour Necrosis Factor Related Weak Inducer of Apoptosis)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TWEAK. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TWEAK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TWEAK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TWEAK. You can calculate the concentration of Human TWEAK in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
96-109
93-104
Average (%)
92
103
99
1:4
Range (%)
86-101
95-101
95-106
Average (%)
94
98
101
1:8
Range (%)
87-100
97-109
86-95
Average (%)
92
101
90
1:16
Range (%)
97-113
94-103
85-95
Average (%)
105
100
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-110
105
EDTA plasma (n=5)
96-107
102
Cell culture media (n=5)
87-98
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.42
60.19
223.79
22.0
53.54
200.93
Standard deviation
1.06
2.88
11.7
1.31
2.66
9.5
C V (%)
5.19
4.78
5.23
5.95
4.97
4.73
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TWEAK concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TWEAK in samples. No significant cross-reactivity or interference between Human TWEAK and analogues was observed.
