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Human Tumor Susceptibility Gene 101 (TSG101) ELISA Kit

SKU:
HUDL03004
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q99816
Sensitivity:
0.105ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
TSG10,VPS23,Tumor Susceptibility Gene 10,ESCRT-I complex subunit TSG101
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Tumor Susceptibility Gene 101 (TSG101) ELISA Kit

The Human TSG101 (Tumor Susceptibility Gene 101) ELISA Kit is specifically designed for the precise measurement of TSG101 levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.TSG101 is an important gene implicated in tumor susceptibility, playing a critical role in regulating cell growth and division.

Dysregulation of TSG101 has been linked to various types of cancer, making it a valuable biomarker for studying cancer development and progression.By utilizing the Human TSG101 ELISA Kit, researchers can effectively analyze TSG101 levels in biological samples, providing insights into the mechanisms underlying cancer and other related diseases. This kit serves as a powerful tool for advancing cancer research and potentially identifying new therapeutic targets.

Product Name:Human Tumor Susceptibility Gene 101 (TSG101) ELISA Kit
Product Code:HUDL03004
Size:96 Assays
Target:TSG101
Synonyms:TSG10, VPS23, Tumor Susceptibility Gene 10, ESCRT-I complex subunit TSG101
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-10296
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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