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Human Tumor Protein P63 (TP63) ELISA Kit

SKU:
HUDL02965
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9H3D4
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
P63,TP73L,CUSP,EEC3,KET,LMS,OFC8,RHS,SHFM4,TP53CP,Tumor Protein p53-Competing Protein,Chronic ulcerative stomatitis protein,Keratinocyte transcription factor
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Tumor Protein P63 (TP63) ELISA Kit

The Human Tumor Protein p63 (TP63) ELISA Kit is specifically designed for the precise measurement of TP63 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.TP63 is a vital protein associated with tumor development, cell differentiation, and epithelial cell integrity. Its dysregulation has been implicated in various cancers, including squamous cell carcinomas and other malignancies.

Therefore, the TP63 ELISA kit serves as a valuable tool for investigating TP63 expression levels in different disease states and potential therapeutic interventions.With its reliable performance and broad applicability, the Human Tumor Protein p63 (TP63) ELISA Kit is an indispensable resource for researchers studying cancer biology, biomarker discovery, and therapeutic developments in the field of oncology.

Product Name:Human Tumor Protein P63 (TP63) ELISA Kit
Product Code:HUDL02965
Size:96 Assays
Target:TP63
Synonyms:P63, TP73L, CUSP, EEC3, KET, LMS, OFC8, RHS, SHFM4, TP53CP, Tumor Protein p53-Competing Protein, Chronic ulcerative stomatitis protein, Keratinocyte transcription factor
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-9994
Heparin plasma (n=5)91-10196
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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