Human Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) ELISA Kit
The Human Tumor Necrosis Factor Receptor Superfamily Member 1B (TNFRSF1B) ELISA Kit is specifically designed for the accurate measurement of TNFRSF1B levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.TNFRSF1B, also known as TNF receptor 2, is a key receptor in the tumor necrosis factor pathway, involved in regulating immune responses and inflammation. Dysregulation of TNFRSF1B has been implicated in various diseases, including autoimmune disorders, inflammatory conditions, and cancer.
Therefore, measuring TNFRSF1B levels can provide valuable insight into disease mechanisms and potential therapeutic targets.With its advanced technology and user-friendly format, the Human TNFRSF1B ELISA Kit is an invaluable tool for researchers studying the role of TNFRSF1B in health and disease. Get accurate and reproducible results with this innovative kit and advance your research with confidence.
Product Name:
Human Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
