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Human Tumor Necrosis Factor Ligand Superfamily, Member 7 (TNFSF7) ELISA Kit

SKU:
HUDL02942
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P32970
Sensitivity:
0.056ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD70,CD27L,CD27LG,TNFSF7,CD27 Ligand
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Human Tumor Necrosis Factor Ligand Superfamily, Member 7 (TNFSF7) ELISA Kit

The Human Tumor Necrosis Factor Ligand Superfamily Member 7 (TNFSF7) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of TNFSF7 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a wide range of research applications.TNFSF7, also known as CD70, is a critical factor in the TNF superfamily that plays a key role in immune responses and inflammation. It is involved in the activation, proliferation, and differentiation of immune cells, making it a valuable biomarker for studying diseases such as cancer, autoimmune disorders, and infectious diseases.

By using the Human TNFSF7 ELISA kit, researchers can gain valuable insights into the role of TNFSF7 in disease pathology and potentially develop novel therapeutic approaches. This kit is easy to use and provides accurate measurements of TNFSF7 levels, making it a valuable tool for advancing research in immunology and oncology.

Product Name:Human Tumor Necrosis Factor Ligand Superfamily, Member 7 (TNFSF7) ELISA Kit
Product Code:HUDL02942
Size:96 Assays
Target:TNFSF7
Synonyms:CD70, CD27L, CD27LG, TNFSF7, CD27 Ligand
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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