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Human Tubulin Alpha 4A (TUBa4A) ELISA Kit

SKU:
HUDL03016
Product Type:
ELISA Kit
Sensitivity:
0.058ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
TUBA1,Testis Specific Tubulin Alpha 1,Alpha-tubulin 1,Tubulin alpha-1 chain,Tubulin H2-alpha
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Human Tubulin Alpha 4A (TUBa4A) ELISA Kit

The Human Tubulin Alpha 4A (TUBA4A) ELISA Kit is a highly reliable and sensitive assay designed for the accurate quantification of TUBA4A levels in human samples such as serum, plasma, and cell culture supernatants. TUBA4A is a key protein involved in microtubule formation and cellular structure, playing a critical role in cell division, intracellular transport, and cell signaling pathways. With its high specificity and precision, this ELISA kit provides researchers with dependable and reproducible results for a variety of research applications, including studies on cancer, neurodegenerative diseases, and developmental biology.

By accurately measuring TUBA4A levels, researchers can gain valuable insights into the role of this protein in various physiological processes and disease states, paving the way for potential therapeutic interventions and diagnostic applications.

Product Name:Human Tubulin Alpha 4A (TUBa4A) ELISA Kit
Product Code:HUDL03016
Size:96 Assays
Target:TUBa4A
Synonyms:TUBA1, Testis Specific Tubulin Alpha 1, Alpha-tubulin 1, Tubulin alpha-1 chain, Tubulin H2-alpha
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-9894
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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