The Human TTR (Transthyretin) ELISA Kit is a precision assay developed for the quantitative measurement of transthyretin levels in human biological samples. Transthyretin, a transport protein primarily synthesized in the liver, contributes to the transport of thyroxine and retinol-binding protein in the bloodstream. Alterations in transthyretin levels have been associated with various health conditions, including familial amyloidosis, senile systemic amyloidosis, and various neuropathies, making it a valuable biomarker for diagnosing and monitoring these disorders. This ELISA kit provides a reliable platform for researchers and clinicians to assess transthyretin concentrations accurately, aiding in disease diagnosis, prognosis, and therapeutic monitoring.
By quantifying transthyretin levels, researchers can gain insights into systemic amyloidosis, neurodegenerative diseases, and other conditions related to transthyretin dysfunction. With exceptional sensitivity and specificity, the Human TTR ELISA Kit ensures precise and reproducible results. Manufactured under stringent quality control standards, this kit offers robust performance and user-friendly operation, making it an ideal choice for research studies focused on transthyretin-related diseases, clinical diagnostics, and personalized medicine approaches.
Product Name:
Human TTR (Transthyretin) ELISA Kit
SKU:
AEES00657
Target:
Human TTR (Transthyretin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.14 ng/mL
Detection range:
0.23-15 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TTR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TTR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TTR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TTR. You can calculate the concentration of Human TTR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-108
86-99
96-109
Average (%)
99
91
102
1:4
Range (%)
94-111
84-96
94-107
Average (%)
101
89
100
1:8
Range (%)
92-105
82-94
99-115
Average (%)
99
89
105
1:16
Range (%)
90-102
87-99
96-110
Average (%)
95
93
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.82
2.32
5.57
0.75
2.16
5.49
Standard deviation
0.04
0.13
0.19
0.04
0.12
0.21
C V (%)
5.45
5.7
3.45
5.2
5.5
3.86
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TTR concentrations in .
Specificity:
This kit recognizes Human TTR in samples. No significant cross-reactivity or interference between Human TTR and analogues was observed.
