Human TSN Recombinant Protein (RPPB5030)
- SKU:
- RPPB5030
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q15631
Description
| Product Name: | Human TSN Recombinant Protein |
| Product Code: | RPPB5030 |
| Size: | 20µg |
| Species: | Human |
| Target: | TSN |
| Synonyms: | Translin, TRSLN, BCLF-1, REHF-1, RCHF1, TBRBP, Recombination Hotspot-binding Protein, Recombination Hotspot Associated factor. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The TSN protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 100mM NaCl, and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MSVSEIFVEL QGFLAAEQDI REEIRKVVQS LEQTAREILT LLQGVHQGAG FQDIPKRCLK AREHFGTVKT HLTSLKTKFP AEQYYRFHEH WRFVLQRLVF LAAFVVYLET ETLVTREAVT EILGIEPDRE KGFHLDVEDY LSGVLILASE LSRLSVNSVT AGDYSRPLHI STFINELDSG FRLLNLKNDS LRKRYDGLKY DVKKVEEVVY DLSIRGFNKE TAAACVEK |
Translin is a DNA and RNA binding protein that identifies specifically preserved target sequences at the breakpoint junction of chromosomal translocations. TSN forms a ring-shaped configuration, that is in charge of DNA binding, and in addition has a leucine zipper motif, which is believed to assist TSN to form dimers. TSN exports specific mRNAs out of the nucleus, reinforced by its localization in both the nuclei and cytoplasm of neurons, and regulates their translation.
TSN produced in E.Coli is a single, non-glycosylated polypeptide chain containing 228 amino acids (1-228a.a.) and having a molecular mass of 26.1kDa. TSN is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TSN: DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translocations, mostly involving immunoglobulin (Ig)/T-cell receptor gene segments. Seems to recognize single-stranded DNA ends generated by staggered breaks occurring at recombination hot spots. Belongs to the translin family. |
| UniProt Protein Details: | Protein type:DNA-binding; Nuclear receptor co-regulator; EC 3.1.-.- Chromosomal Location of Human Ortholog: 2q21.1 Cellular Component: cytosol Molecular Function:DNA binding; protein binding Biological Process: DNA recombination; RNA-mediated gene silencing |
| NCBI Summary: | This gene encodes a DNA-binding protein which specifically recognizes conserved target sequences at the breakpoint junction of chromosomal translocations. Translin polypeptides form a multimeric structure that is responsible for its DNA-binding activity. Recombination-associated motifs and translin-binding sites are present at recombination hotspots and may serve as indicators of breakpoints in genes which are fused by translocations. These binding activities may play a crucial role in chromosomal translocation in lymphoid neoplasms. This protein encoded by this gene, when complexed with translin-associated protein X, also forms a Mg ion-dependent endoribonuclease that promotes RNA-induced silencing complex (RISC) activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2012] |
| UniProt Code: | Q15631 |
| NCBI GenInfo Identifier: | 6136060 |
| NCBI Gene ID: | 7247 |
| NCBI Accession: | Q15631.1 |
| UniProt Secondary Accession: | Q15631,Q5U0K7, B7Z3X8, |
| UniProt Related Accession: | Q15631 |
| Molecular Weight: | 17,279 Da |
| NCBI Full Name: | Translin |
| NCBI Synonym Full Names: | translin |
| NCBI Official Symbol: | TSN�� |
| NCBI Official Synonym Symbols: | C3PO; RCHF1; TBRBP; TRSLN; BCLF-1; REHF-1�� |
| NCBI Protein Information: | translin |
| UniProt Protein Name: | Translin |
| UniProt Synonym Protein Names: | Component 3 of promoter of RISC; C3PO |
| Protein Family: | Translin |
| UniProt Gene Name: | TSN�� |
| UniProt Entry Name: | TSN_HUMAN |
