Human TSLP (Thymic Stromal Lymphopoietin ) CLIA Kit
The Human TSLP (Thymic Stromal Lymphopoietin) CLIA Kit is specifically designed for the precise measurement of TSLP levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and accuracy, this kit ensures dependable and consistent results, making it an excellent tool for a variety of research purposes.TSLP is a critical cytokine that plays a key role in immune responses, inflammation, and the development of allergic diseases. It is involved in the regulation of T cell functions, dendritic cell maturation, and the activation of mast cells, making it a valuable biomarker for studying immune-related disorders and potential therapeutic interventions.
With the Human TSLP CLIA Kit, researchers can gain valuable insights into the role of TSLP in various physiological and pathological processes, paving the way for advancements in the fields of immunology, allergy, and inflammatory diseases. Discover the power of accurate TSLP measurement with this advanced assay kit from AssayGenie.
Product Name:
Human TSLP (Thymic Stromal Lymphopoietin ) CLIA Kit
SKU:
HUES00909
Target:
Human TSLP (Thymic Stromal Lymphopoietin )
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TSLP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TSLP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TSLP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TSLP. You can calculate the concentration of Human TSLP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
89-101
86-101
Average (%)
98
94
93
1:4
Range (%)
95-112
92-104
93-108
Average (%)
102
97
99
1:8
Range (%)
85-98
99-111
89-100
Average (%)
92
104
95
1:16
Range (%)
88-102
101-115
89-101
Average (%)
95
108
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
102
EDTA plasma (n=5)
90-104
97
Cell culture media (n=5)
89-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
36.53
106.91
390.11
36.2
108.8
363.57
Standard deviation
3.34
10.48
38.04
3.17
8.54
39.88
C V (%)
9.14
9.8
9.75
8.76
7.85
10.97
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TSLP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TSLP in samples. No significant cross-reactivity or interference between Human TSLP and analogues was observed.
