The TSH ELISA Kit is designed for the quantitative detection of Thyroid Stimulating Hormone (TSH) levels in various human biological samples.
TSH is a critically important hormone involved in the regulation of metabolic processes, integral in the stimulation of thyroid hormone production and release. It plays a significant role in the body's metabolic activities and disturbances in its levels can lead to various conditions like hyperthyroidism or hypothyroidism. Accurate measurement of TSH is imperative for understanding its role in metabolic regulation and for the development of effective therapeutic strategies. Assay Genie's TSH ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit ensures robust performance and is easy to use, making it an excellent choice for both research and clinical applications. Trust Assay Genie's TSH ELISA Kit for accurate and reliable quantification of this crucial biomarker in your studies.
Product Name:
Human TSH (Thyroid Stimulating Hormone) ELISA Kit
SKU:
AEES00037
Target:
Human TSH (Thyroid Stimulating Hormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 μIU/mL
Detection range:
0.31-20 μIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TSH. You can calculate the concentration of Human TSH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
86-94
96-109
Average (%)
102
90
103
1:4
Range (%)
90-103
89-99
83-96
Average (%)
97
93
91
1:8
Range (%)
96-109
99-106
90-105
Average (%)
102
103
97
1:16
Range (%)
87-99
99-108
88-101
Average (%)
95
103
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-109
102
EDTA plasma (n=5)
92-99
96
Cell culture media (n=5)
95-105
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.92
2.42
8.6
0.99
2.15
8.4
Standard deviation
0.05
0.11
0.38
0.05
0.1
0.4
C V (%)
5.43
4.55
4.42
5.05
4.65
4.76
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TSH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TSH in samples. No significant cross-reactivity or interference between Human TSH and analogues was observed.
