null

Human Tryptophan Hydroxylase 2 (TPH2) ELISA Kit

SKU:
HUDL02968
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8IWU9
Sensitivity:
0.127ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
NTPH,Neuronal tryptophan hydroxylase,Tryptophan 5-monooxygenase 2
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Tryptophan Hydroxylase 2 (TPH2) ELISA Kit

The Human Tryptophan Hydroxylase 2 (TPH2) ELISA Kit is a highly reliable and accurate tool for the quantitative measurement of TPH2 levels in human samples such as serum, plasma, and cell culture supernatants. TPH2 is an essential enzyme involved in the serotonin biosynthesis pathway, playing a crucial role in regulating mood and behavior.This ELISA kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.

By measuring TPH2 levels, researchers can gain valuable insights into the role of serotonin synthesis in various physiological and pathological processes, including psychiatric disorders, neurodegenerative diseases, and drug responses.Overall, the Human TPH2 ELISA Kit is a valuable resource for researchers seeking to study the mechanisms underlying serotonin regulation and its implications for human health and disease.

Product Name:Human Tryptophan Hydroxylase 2 (TPH2) ELISA Kit
Product Code:HUDL02968
Size:96 Assays
Target:TPH2
Synonyms:NTPH, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products