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Human Tryptophan Hydroxylase 1 (TPH1) ELISA Kit

SKU:
HUDL02967
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P17752
Sensitivity:
0.125ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
TPH,TPRH,TRPH,Tryptophan 5-Monooxygenase
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Tryptophan Hydroxylase 1 (TPH1) ELISA Kit

The Human Tryptophan Hydroxylase-1 (TPH1) ELISA Kit is a precise tool for the quantitative detection of TPH1 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it suitable for a variety of research purposes.TPH1 is a key enzyme involved in the biosynthesis of serotonin, a neurotransmitter that plays a critical role in regulating mood, behavior, and cognition.

Dysregulation of TPH1 activity has been linked to various psychiatric disorders, making it a valuable biomarker for understanding and potentially treating these conditions.By utilizing the Human TPH1 ELISA Kit, researchers can gain valuable insights into the role of TPH1 in serotonin production and its implications for mental health. This kit is an essential tool for investigating the mechanisms underlying psychiatric disorders and developing novel therapeutic strategies.

Product Name:Human Tryptophan Hydroxylase 1 (TPH1) ELISA Kit
Product Code:HUDL02967
Size:96 Assays
Target:TPH1
Synonyms:TPH, TPRH, TRPH, Tryptophan 5-Monooxygenase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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