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Human Trypsinogen Activation Peptide (TAP) ELISA Kit

SKU:
HUDL02829
Product Type:
ELISA Kit
Sensitivity:
0.08nmol/L
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Human Trypsinogen Activation Peptide (TAP) ELISA Kit

The Human Trypsinogen Activation Peptide (TAP) ELISA Kit is specifically designed for the accurate and precise detection of human trypsinogen activation peptide levels in serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.The trypsinogen activation peptide is a key component in the activation of trypsinogen, an important enzyme involved in protein digestion.

Dysregulation of trypsinogen activation peptide levels has been linked to various gastrointestinal disorders, making it a valuable biomarker for studying and understanding these conditions.With easy-to-follow instructions and a user-friendly format, the Human TAP ELISA Kit is a valuable tool for researchers looking to investigate the role of trypsinogen activation peptide in health and disease. Order yours today and take your research to the next level.

Product Name:Human Trypsinogen Activation Peptide (TAP) ELISA Kit
Product Code:HUDL02829
Size:96 Assays
Target:TAP
Detection Method:Competition
Range:0.25-16nmol/L
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.Aspirate and wash 3 times
4.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.Aspirate and wash 5 times
6.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.Add 50µL Stop Solution. Read at 450 nm immediately.

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