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Human Tropomyosin 3 (TPM3) ELISA Kit

SKU:
HUDL02973
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P06753
Sensitivity:
0.126ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
NEM1,TRK,OK/SW-cl.5,TM-5,TM3,TM30,TM30nm,TPMsk3,Hscp30,Gamma-tropomyosin
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Tropomyosin 3 (TPM3) ELISA Kit

The Human Tropomyosin-3 (TPM3) ELISA Kit is a highly reliable and sensitive tool for the accurate measurement of TPM3 levels in human samples including serum, plasma, and cell culture supernatants. This kit is designed to deliver consistent and reproducible results, making it an ideal choice for various research applications.TPM3 is a vital protein involved in regulating muscle contraction and cell movement. Abnormal TPM3 levels have been linked to various diseases such as cancer, heart disease, and neuromuscular disorders.

Therefore, the TPM3 ELISA Kit serves as a valuable tool for studying these conditions and potentially developing new therapeutic interventions.Overall, the Human TPM3 ELISA Kit from Assay Genie offers researchers a powerful and precise method for quantifying TPM3 levels, ultimately advancing our understanding of its role in health and disease.

Product Name:Human Tropomyosin 3 (TPM3) ELISA Kit
Product Code:HUDL02973
Size:96 Assays
Target:TPM3
Synonyms:NEM1, TRK, OK/SW-cl.5, TM-5, TM3, TM30, TM30nm, TPMsk3, Hscp30, Gamma-tropomyosin
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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