null

Human Tropomyosin 2 Beta (TPM2) ELISA Kit

SKU:
HUDL02972
Product Type:
ELISA Kit
Sensitivity:
0.247ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
AMCD1,DA1,TMSB,Arthrogryposis Multiplex Congenital,Distal,Type 1
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Tropomyosin 2 Beta (TPM2) ELISA Kit

The Human Tropomyosin 2 Beta (TPM2) ELISA Kit is specifically designed for the precise measurement of TPM2 levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it a reliable tool for various research applications.TPM2 is a critical protein that plays a key role in regulating muscle contractions and maintaining muscle structure. Dysregulation of TPM2 has been linked to various muscle-related disorders and diseases, making it a valuable biomarker for studying these conditions and developing potential treatment strategies.

This ELISA kit provides researchers with a powerful tool to study the role of TPM2 in muscle function and disease pathology. Its user-friendly design and robust performance make it an essential asset for any laboratory working to advance our understanding of muscle-related conditions.

Product Name:Human Tropomyosin 2 Beta (TPM2) ELISA Kit
Product Code:HUDL02972
Size:96 Assays
Target:TPM2
Synonyms:AMCD1, DA1, TMSB, Arthrogryposis Multiplex Congenital,Distal,Type 1
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products