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Human Tripeptidyl Peptidase I (TPP1) ELISA Kit

SKU:
HUDL02976
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O14773
Sensitivity:
0.063ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CLN2,GIG1,LPIC,Tripeptidyl aminopeptidase,Lysosomal pepstatin-insensitive protease,Ceroid-Lipofuscinosis,Neuronal 2,Late Infantile,Cell growth-inhibiting gene 1 protein
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

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Human Tripeptidyl Peptidase I (TPP1) ELISA Kit

The Human Tripeptidyl Peptidase I (TPP1) ELISA Kit is a reliable and accurate method for detecting TPP1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Tripeptidyl Peptidase I (TPP1) is an important enzyme involved in lysosomal function and protein degradation. Dysregulation of TPP1 has been linked to neurodegenerative diseases such as Batten disease, highlighting its significance as a potential biomarker for studying these conditions and developing therapeutic strategies.

With the Human TPP1 ELISA Kit, researchers can confidently analyze TPP1 levels in biological samples, contributing to a better understanding of its role in disease pathogenesis and potential treatment options.

Product Name:Human Tripeptidyl Peptidase I (TPP1) ELISA Kit
Product Code:HUDL02976
Size:96 Assays
Target:TPP1
Synonyms:CLN2, GIG1, LPIC, Tripeptidyl aminopeptidase, Lysosomal pepstatin-insensitive protease, Ceroid-Lipofuscinosis,Neuronal 2,Late Infantile, Cell growth-inhibiting gene 1 protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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