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Human Transportin 2 (TNPO2) ELISA Kit(

SKU:
HUDL02955
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O14787
Sensitivity:
0.121ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
IPO3,KPNB2B,TRN2,Importin 3,Karyopherin Beta 2b
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Transportin 2 (TNPO2) ELISA Kit (

The Human Transportin 2 (TNPO2) ELISA Kit is a reliable tool for detecting levels of transportin 2 in human samples. This kit offers high sensitivity and specificity, allowing for accurate and reproducible results in serum, plasma, and cell culture supernatants.Transportin 2 plays a key role in nuclear import processes, facilitating the transport of proteins into the nucleus. Dysregulation of transportin 2 has been linked to various diseases, including viral infections and neurodegenerative disorders.

By accurately measuring transportin 2 levels, researchers can gain insights into disease mechanisms and potential therapeutic targets.The Human Transportin 2 ELISA Kit is designed for easy and efficient use in a wide range of research applications, making it a valuable tool for studying the role of transportin 2 in health and disease.

Product Name:Human Transportin 2 (TNPO2) ELISA Kit(
Product Code:HUDL02955
Size:96 Assays
Target:TNPO2
Synonyms:IPO3, KPNB2B, TRN2, Importin 3, Karyopherin Beta 2b
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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