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Human Transmembrane Protein 2 Like Protein (TMEM2L) ELISA Kit

SKU:
HUDL02923
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8WUJ3
Sensitivity:
0.052ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CEMIP,CCSP1,KIAA1199,Cell Migration Inducing Protein, Hyaluronan Binding
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Human Transmembrane Protein 2 Like Protein (TMEM2L) ELISA Kit

The Human Transmembrane Protein 2-Like Protein (TMEM2L) ELISA Kit is specifically designed for the accurate detection of TMEM2L levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.TMEM2L is an important transmembrane protein that plays a role in various cellular processes, including cell signaling and membrane transport. Studies have shown that dysregulation of TMEM2L expression can be associated with certain diseases, making it a valuable biomarker for research in areas such as cancer, neurological disorders, and immune responses.

With its user-friendly protocol and high-quality components, the TMEM2L ELISA Kit from Assay Genie provides researchers with a powerful tool for studying the function and regulation of TMEM2L in health and disease. Order yours today to advance your research in the field of transmembrane protein biology.

Product Name:Human Transmembrane Protein 2 Like Protein (TMEM2L) ELISA Kit
Product Code:HUDL02923
Size:96 Assays
Target:TMEM2L
Synonyms:CEMIP, CCSP1, KIAA1199, Cell Migration Inducing Protein, Hyaluronan Binding
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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