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Human Transketolase Like Protein 1 (TKTL1) ELISA Kit

SKU:
HUDL02908
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P51854
Sensitivity:
0.062ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
TKR,TKT2,TK 2,Transketolase-related protein,Transketolase 2
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Transketolase Like Protein 1 (TKTL1) ELISA Kit

The Human Transketolase Like Protein 1 (TKTL1) ELISA Kit is specifically designed for the accurate and reliable detection of TKTL1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results that are essential for a wide range of research applications.TKTL1 is a key protein that plays a crucial role in various cellular processes, including energy metabolism and cell proliferation.

Aberrant expression of TKTL1 has been associated with cancer progression, making it a valuable biomarker for studying cancer biology and potential therapeutic interventions.With its advanced technology and easy-to-use format, the Human TKTL1 ELISA Kit is an essential tool for researchers and clinicians looking to investigate the role of TKTL1 in cancer and other diseases, paving the way for new diagnostic and treatment strategies.

Product Name:Human Transketolase Like Protein 1 (TKTL1) ELISA Kit
Product Code:HUDL02908
Size:96 Assays
Target:TKTL1
Synonyms:TKR, TKT2, TK 2, Transketolase-related protein, Transketolase 2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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