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Human Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1) ELISA Kit

SKU:
HUDL03000
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8NER1
Sensitivity:
0.112ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
VR1,OTRPC1,Vanilloid Receptor Subtype 1,Capsaicin receptor,Osm-9-like TRP channel 1,Vanilloid receptor 1
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1) ELISA Kit

The Human Transient Receptor Potential Cation Channel Subfamily V Member 1 (TRPV1) ELISA Kit is specifically designed for the accurate detection of TRPV1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications. TRPV1 is a key protein involved in pain sensation and inflammation, making it a crucial target for studies related to chronic pain conditions, neurological disorders, and inflammatory diseases.

By accurately measuring TRPV1 levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic strategies. With its user-friendly protocol and reliable performance, the Human TRPV1 ELISA Kit is an essential tool for researchers looking to advance their understanding of TRPV1 biology and its implications for human health.

Product Name:Human Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1) ELISA Kit
Product Code:HUDL03000
Size:96 Assays
Target:TRPV1
Synonyms:VR1, OTRPC1, Vanilloid Receptor Subtype 1, Capsaicin receptor, Osm-9-like TRP channel 1, Vanilloid receptor 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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