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Human Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6) ELISA Kit

SKU:
HUDL02995
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Y210
Sensitivity:
0.129ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
FSGS2,TRP6,Transient receptor protein 6
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6) ELISA Kit

The Human Transient Receptor Potential Cation Channel Subfamily C Member 6 (TRPC6) ELISA Kit is a highly specific and sensitive assay designed for the detection of TRPC6 levels in human serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an ideal tool for research applications in studying the role of TRPC6 in various physiological and pathological processes.TRPC6 is a member of the TRP superfamily of cation channels, which play a crucial role in cellular calcium signaling and regulation.

Dysregulation of TRPC6 has been implicated in various diseases, including cardiovascular disorders, renal diseases, and neurological disorders. By measuring TRPC6 levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.Overall, the Human TRPC6 ELISA Kit offers a reliable and efficient method for quantifying TRPC6 levels, making it a valuable tool for advancing research in the field of calcium signaling and channelopathies.

Product Name:Human Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6) ELISA Kit
Product Code:HUDL02995
Size:96 Assays
Target:TRPC6
Synonyms:FSGS2, TRP6, Transient receptor protein 6
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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