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Human Transient Receptor Potential Cation Channel Subfamily A, Member 1 (TRPA1) ELISA Kit

SKU:
HUDL02994
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O75762
Sensitivity:
0.214ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
ANKTM1,Ankyrin-like with transmembrane domains protein 1,Transformation-sensitive protein p120
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Transient Receptor Potential Cation Channel Subfamily A, Member 1 (TRPA1) ELISA Kit

The Human Transient Receptor Potential Cation Channel Subfamily A Member 1 (TRPA1) ELISA Kit is a powerful tool for the precise measurement of TRPA1 levels in human samples such as serum, plasma, and cell culture supernatants. With a focus on high sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable resource for a variety of research endeavors.TRPA1 is a key player in sensory functions, particularly in detecting various chemical and mechanical stimuli in the body.

Dysregulation of TRPA1 has been implicated in conditions like chronic pain, inflammatory disorders, and respiratory diseases, highlighting its significance as a potential therapeutic target and diagnostic marker.By utilizing the Human TRPA1 ELISA Kit, researchers can delve deeper into the complexities of TRPA1 biology and its impact on human health, ultimately paving the way for innovative interventions and treatment strategies.

Product Name:Human Transient Receptor Potential Cation Channel Subfamily A, Member 1 (TRPA1) ELISA Kit
Product Code:HUDL02994
Size:96 Assays
Synonyms:ANKTM1, Ankyrin-like with transmembrane domains protein 1, Transformation-sensitive protein p120
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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