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Human Transglutaminase 6 (TGM6) ELISA Kit

SKU:
HUDL02879
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O95932
Sensitivity:
0.116ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
TGM3L,TGY,SCA35,Transglutaminase 3-Like,Spinocerebellar Ataxia 35,Transglutaminase Y,Protein-glutamine gamma-glutamyltransferase 6
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Transglutaminase 6 (TGM6) ELISA Kit

The Human Transglutaminase-6 (TGM6) ELISA Kit is designed for the accurate detection of TGM6 levels in human serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications.Transglutaminase-6 is a key enzyme involved in various cellular processes, including protein cross-linking and cell signaling.

Dysregulation of TGM6 has been implicated in autoimmune diseases such as celiac disease and neurological disorders like ataxia, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its easy-to-use format and precise performance, the Human Transglutaminase-6 ELISA Kit is a valuable tool for researchers exploring the role of TGM6 in human health and disease.

Product Name:Human Transglutaminase 6 (TGM6) ELISA Kit
Product Code:HUDL02879
Size:96 Assays
Target:TGM6
Synonyms:TGM3L, TGY, SCA35, Transglutaminase 3-Like, Spinocerebellar Ataxia 35, Transglutaminase Y, Protein-glutamine gamma-glutamyltransferase 6
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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