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Human Transforming Growth Factor Beta Stimulated Protein Clone 22 (TSC22) ELISA Kit

SKU:
HUDL03003
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q15714
Sensitivity:
0.113ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
TSC22D1,TGFB1I4,TSC22,hucep-2,Cerebral protein 2,Transforming Growth Factor Beta 1 Induced Transcript 4,TSC22 Domain Family,Member 1
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$719
Frequently bought together:

Description

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Human Transforming Growth Factor Beta Stimulated Protein Clone 22 (TSC22) ELISA Kit

The Human Transforming Growth Factor-Beta Stimulated Protein Clone 22 (TSC22) ELISA Kit is designed for the precise measurement of TSC22 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.TSC22 is a key protein regulated by transforming growth factor-beta (TGF-β) that plays a vital role in various cellular processes, including cell growth, differentiation, and apoptosis.

Abnormalities in TSC22 expression have been linked to conditions such as cancer, inflammation, and fibrosis, making it a valuable biomarker for studying disease pathogenesis and potential therapeutic targets.Researchers and clinicians can rely on the Human TSC22 ELISA Kit for robust and reliable detection of this important protein, enabling groundbreaking discoveries and advancements in biomedical research.

Product Name:Human Transforming Growth Factor Beta Stimulated Protein Clone 22 (TSC22) ELISA Kit
Product Code:HUDL03003
Size:96 Assays
Target:TSC22
Synonyms:TSC22D1, TGFB1I4, TSC22, hucep-2, Cerebral protein 2, Transforming Growth Factor Beta 1 Induced Transcript 4, TSC22 Domain Family,Member 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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