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Human Transforming Growth Factor Alpha (TGFa) ELISA Kit

SKU:
HUDL02867
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P01135
Sensitivity:
12.78pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
ETGF,TGF-A,Protransforming Growth Factor alpha,EGF-Like TGF
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

system_update_altDatasheet

Human Transforming Growth Factor Alpha (TGFa) ELISA Kit

The Human Transforming Growth Factor Alpha (TGFA) ELISA Kit is specifically designed for the quantitative measurement of TGFA levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications.TGFA is a key growth factor that plays a critical role in cell growth, differentiation, and development. It is involved in various physiological processes, including wound healing, tissue regeneration, and embryonic development.

Dysregulation of TGFA has been implicated in various diseases, such as cancer, inflammatory disorders, and fibrotic diseases, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.With its precise measurement capabilities and broad applicability, the Human TGFA ELISA Kit is an essential tool for researchers studying the role of TGFA in disease pathogenesis and potential therapeutic interventions.

Product Name:Human Transforming Growth Factor Alpha (TGFa) ELISA Kit
Product Code:HUDL02867
Size:96 Assays
Target:TGFa
Synonyms:ETGF, TGF-A, Protransforming Growth Factor alpha, EGF-Like TGF
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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