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Human Transferrin (TRF) ELISA Kit

SKU:
HUDL02990
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P02787
Sensitivity:
0.67ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
TF,Siderophilin,Serotransferrin,Beta-1 metal-binding globulin
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

system_update_altDatasheet

Human Transferrin (TRF) ELISA Kit

The Human Transferrin (TRF) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of transferrin levels in human serum, plasma, and cell culture supernatants. Transferrin is a key protein involved in iron transport and metabolism, essential for proper functioning of the body's cells and organs.This ELISA kit offers reliable and reproducible results, making it ideal for a wide range of research applications in areas such as iron metabolism, anemia, and liver diseases.

By measuring transferrin levels, researchers can gain valuable insights into iron deficiency and overload conditions, as well as monitor treatment responses in patients with related disorders.Overall, the Human Transferrin (TRF) ELISA Kit provides a valuable tool for studying iron metabolism and related conditions, contributing to advancements in the development of potential therapies for various diseases.

Product Name:Human Transferrin (TRF) ELISA Kit
Product Code:HUDL02990
Size:96 Assays
Target:TRF
Synonyms:TF, Siderophilin, Serotransferrin, Beta-1 metal-binding globulin
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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