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Human Toll Like Receptor 8 (TLR8) ELISA Kit

SKU:
HUDL02920
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9NR97
Sensitivity:
0.108ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD288
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Toll Like Receptor 8 (TLR8) ELISA Kit

The Human Toll-like Receptor 8 (TLR8) ELISA Kit is a powerful tool for researchers looking to accurately measure TLR8 levels in human samples. This kit boasts high sensitivity and specificity, guaranteeing precise and consistent results across serum, plasma, and cell culture supernatants.TLR8 is a key component of the innate immune system, recognizing viral and bacterial nucleic acids and triggering immune responses. Its dysregulation has been implicated in various diseases, including autoimmune disorders and viral infections.

By accurately measuring TLR8 levels, researchers can gain valuable insights into immune system function and develop new therapeutic strategies.Whether investigating immune responses, studying infectious diseases, or exploring novel drug targets, the Human TLR8 ELISA Kit is an indispensable tool for advancing research in immunology and beyond.

Product Name:Human Toll Like Receptor 8 (TLR8) ELISA Kit
Product Code:HUDL02920
Size:96 Assays
Target:TLR8
Synonyms:CD288
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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