Human TNNT1 (Troponin T Type 1, Slow Skeletal) ELISA Kit
The Human TNNT1 (Troponin T Type 1 Slow Skeletal) ELISA Kit is specifically designed for the accurate measurement of TNNT1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.TNNT1 is a key protein involved in muscle contraction and is predominantly found in slow-twitch skeletal muscle fibers. Abnormal levels of TNNT1 have been associated with various skeletal muscle disorders, making it a valuable biomarker for studying these conditions and developing potential therapies.
With its user-friendly format and quality components, the Human TNNT1 ELISA Kit is an indispensable tool for researchers and clinicians interested in investigating the role of TNNT1 in skeletal muscle function and disease. Its versatility and accuracy make it a valuable asset for advancing scientific knowledge and improving patient care.
Product Name:
Human TNNT1 (Troponin T Type 1, Slow Skeletal) ELISA Kit
SKU:
HUES02531
Target:
Human TNNT1 (Troponin T Type 1, Slow Skeletal)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNNT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNNT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNNT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNNT1. You can calculate the concentration of Human TNNT1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-106
87-100
94-106
Average (%)
97
94
99
1:4
Range (%)
92-103
85-97
82-97
Average (%)
97
90
89
1:8
Range (%)
90-103
82-94
82-95
Average (%)
97
89
88
1:16
Range (%)
90-103
85-98
90-103
Average (%)
95
90
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-100
95
EDTA plasma (n=5)
90-103
95
Cell culture media (n=5)
92-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.63
90.62
483.83
49.04
96.14
444.11
Standard deviation
2.82
3.63
26.37
2.95
4.57
17.19
C V (%)
5.46
4.01
5.45
6.02
4.75
3.87
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TNNT1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TNNT1 in samples. No significant cross-reactivity or interference between Human TNNT1 and analogues was observed.
