Human TNNI2 (Troponin I Type 2, Fast Skeletal) ELISA Kit (HUES02493)
- SKU:
- HUES02493
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P48788
- Sensitivity:
- 46.88pg/mL
- Range:
- 78.13-5000pg/mL
- ELISA Type:
- Sandwich
- Reactivity:
- Human
- Sample Type:
- Serum, plasma and other biological fluids
- Research Area:
- Cell Biology
Description
Human TNNI2 (Troponin I Type 2, Fast Skeletal) ELISA Kit
The Human TNNT2 (Troponin I, Type 2, Fast Skeletal) ELISA Kit is a reliable and accurate tool for the measurement of TNNT2 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.TNNT2 is a key protein involved in muscle contraction and plays a critical role in skeletal muscle function. Dysregulation of TNNT2 has been implicated in various muscle disorders and diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.
With its advanced technology and superior performance, the Human TNNT2 ELISA Kit is an essential tool for researchers in the field of muscle biology and physiology. Get reliable and accurate results with this easy-to-use kit for your research needs.
| Assay type: | Sandwich |
| Format: | 96T |
| Assay time: | 4.5h |
| Reactivity: | Human |
| Detection Method: | Colormetric |
| Detection Range: | 78.13-5000 pg/mL |
| Sensitivity: | 46.88 pg/mL |
| Sample Volume Required Per Well: | 100µL |
| Sample Type: | Serum, plasma and other biological fluids |
| Specificity: | This kit recognizes Human TNNI2 in samples. No significant cross-reactivity or interference between Human TNNI2 and analogues was observed. |
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNNI2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNNI2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNNI2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNNI2. The concentration of Human TNNI2 in samples can be calculated by comparing the OD of the samples to the standard curve.
| UniProt Protein Function: | TNNI2: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNI2 are a cause of distal arthrogryposis type 2B (DA2B); also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. Belongs to the troponin I family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 11p15. 5 Cellular Component: troponin complex; cytosol; nucleus Molecular Function:troponin T binding; protein binding; actin binding Biological Process: skeletal muscle contraction; regulation of muscle contraction; positive regulation of transcription, DNA-dependent; muscle filament sliding Disease: Arthrogryposis, Distal, Type 2b |
| NCBI Summary: | This gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies show that this component is also present in vascular smooth muscle and may play a role in regulation of smooth muscle function. In addition to muscle tissues, this protein is found in corneal epithelium, cartilage where it is an inhibitor of angiogenesis to inhibit tumor growth and metastasis, and mammary gland where it functions as a co-activator of estrogen receptor-related receptor alpha. This protein also suppresses tumor growth in human ovarian carcinoma. Mutations in this gene cause myopathy and distal arthrogryposis type 2B. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009] |
| UniProt Code: | P48788 |
| NCBI GenInfo Identifier: | 1351297 |
| NCBI Gene ID: | 7136 |
| NCBI Accession: | P48788. 2 |
| UniProt Secondary Accession: | P48788,A6NIV8, A6NJU5, |
| UniProt Related Accession: | P48788 |
| Molecular Weight: | 182 |
| NCBI Full Name: | Troponin I, fast skeletal muscle |
| NCBI Synonym Full Names: | troponin I type 2 (skeletal, fast) |
| NCBI Official Symbol: | TNNI2 |
| NCBI Official Synonym Symbols: | DA2B; FSSV; fsTnI; AMCD2B |
| NCBI Protein Information: | troponin I, fast skeletal muscle; troponin I fast twitch 2; troponin I, skeletal, fast; troponin I, fast-twitch isoform; troponin I, fast-twitch skeletal muscle isoform |
| UniProt Protein Name: | Troponin I, fast skeletal muscle |
| UniProt Synonym Protein Names: | Troponin I, fast-twitch isoform |
| Protein Family: | Troponin |
| UniProt Gene Name: | TNNI2 |
| UniProt Entry Name: | TNNI2_HUMAN |
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e. g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
| Concentration (pg/mL) | O.D | Average | Corrected |
| 5000 | 2.549 2.585 | 2.567 | 2.476 |
| 2500 | 1.798 1.802 | 1.8 | 1.709 |
| 1250 | 0.988 0.958 | 0.973 | 0.882 |
| 625 | 0.539 0.575 | 0.557 | 0.466 |
| 312.5 | 0.313 0.299 | 0.306 | 0.215 |
| 156.25 | 0.206 0.192 | 0.199 | 0.108 |
| 78.13 | 0.143 0.149 | 0.146 | 0.055 |
| 0 | 0.091 0.091 | 0.091 | -- |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TNNI2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TNNI2 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 232.93 | 557.69 | 2288.37 | 238.35 | 526.10 | 2119.89 |
| Standard deviation | 16.03 | 30.45 | 82.61 | 13.47 | 21.99 | 73.98 |
| C V (%) | 6.88 | 5.46 | 3.61 | 5.65 | 4.18 | 3.49 |
Recovery
The recovery of Human TNNI2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum (n=5) | 84-99 | 91 |
| EDTA plasma (n=5) | 86-100 | 93 |
| Cell culture media (n=5) | 92-106 | 98 |
Linearity
Samples were spiked with high concentrations of Human TNNI2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
| 1:2 | Range (%) | 92-105 | 99-116 | 90-103 |
| Average (%) | 98 | 106 | 97 | |
| 1:4 | Range (%) | 92-107 | 83-95 | 89-103 |
| Average (%) | 98 | 89 | 94 | |
| 1:8 | Range (%) | 88-99 | 81-91 | 88-100 |
| Average (%) | 94 | 86 | 93 | |
| 1:16 | Range (%) | 91-104 | 84-95 | 86-100 |
| Average (%) | 97 | 89 | 93 |
An unopened kit can be stored at 4°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.
| Item | Specifications | Storage |
| Micro ELISA Plate(Dismountable) | 8 wells ×12 strips | -20°C, 6 months |
| Reference Standard | 2 vials | |
| Concentrated Biotinylated Detection Ab (100×) | 1 vial, 120 µL | |
| Concentrated HRP Conjugate (100×) | 1 vial, 120 µL | -20°C(shading light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 4°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
| HRP Conjugate Diluent | 1 vial, 14 mL | |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
| Substrate Reagent | 1 vial, 10 mL | 4°C(shading light) |
| Stop Solution | 1 vial, 10 mL | 4°C |
| Plate Sealer | 5 pieces | |
| Product Description | 1 copy | |
| Certificate of Analysis | 1 copy |
- Set standard, test sample and control (zero) wells on the pre-coated plate and record theirpositions. It is recommended to measure each standard and sample in duplicate. Note: addall solutions to the bottom of the plate wells while avoiding contact with the well walls. Ensuresolutions do not foam when adding to the wells.
- Aliquot 100µl of standard solutions into the standard wells.
- Add 100µl of Sample / Standard dilution buffer into the control (zero) well.
- Add 100µl of properly diluted sample (serum, plasma, tissue homogenates and otherbiological fluids) into test sample wells.
- Cover the plate with the sealer provided in the kit and incubate for 90 min at 37°C.
- Aspirate the liquid from each well, do not wash. Immediately add 100µL of BiotinylatedDetection Ab working solution to each well. Cover the plate with a plate seal and gently mix. Incubate for 1 hour at 37°C.
- Aspirate or decant the solution from the plate and add 350µL of wash buffer to each welland incubate for 1-2 minutes at room temperature. Aspirate the solution from each well andclap the plate on absorbent filter paper to dry. Repeat this process 3 times. Note: a microplatewasher can be used in this step and other wash steps.
- Add 100µL of HRP Conjugate working solution to each well. Cover with a plate seal andincubate for 30 min at 37°C.
- Aspirate or decant the solution from each well. Repeat the wash process for five times asconducted in step 7.
- Add 90µL of Substrate Reagent to each well. Cover with a new plate seal and incubate forapproximately 15 min at 37°C. Protect the plate from light. Note: the reaction time can beshortened or extended according to the actual color change, but not by more than 30min.
- Add 50 µL of Stop Solution to each well. Note: Adding the stop solution should be done inthe same order as the substrate solution.
- Determine the optical density (OD value) of each well immediately with a microplate readerset at 450 nm.
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