Human TNFRSF10A Fc Recombinant Protein (RPPB1025)
- SKU:
- RPPB1025
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O00220
- Research Area:
- Cytokines
Description
| Product Name: | Human TNFRSF10A Fc Recombinant Protein |
| Product Code: | RPPB1025 |
| Size: | 50µg |
| Species: | Human |
| Target: | TNFRSF10A Fc |
| Synonyms: | Tumor Necrosis Factor Receptor Superfamily Member 10a, TRAILR1, APO2, DR4, TNF-Related Apoptosis-Inducing Ligand Receptor 1, Death Receptor 4, TRAIL Receptor 1, TRAIL-R1, Tumor Necrosis Factor Receptor Superfamily Member 10a Variant 2, Tumor Necrosis Factor Receptor Superfamily Member 10A, Cytotoxic TRAIL Receptor, CD261 Antigen, TRAILR-1, CD261, TNFRSF10A. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered solution in PBS. |
| Solubility: | It is recommended to reconstitute the lyophilized TNFRSF10A in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized TNFRSF10A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TRAIL-R1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by�SDS-PAGE. |
| Amino Acid Sequence: | ASGTEAAAAT PSKVWGSSAG RIEPRGGGRG ALPTSMGQHG PSARARAGRA PGPRPAREAS PRLRVHKTFK FVVVGVLLQV VPSSAATIKL HDQSIGTQQW EHSPLGELCP PGSHRSERPG ACNRCTEGVG YTNASNNLFA CLPCTACKSD EEERSPCTTT RNTACQCKPG TFRNDNSAEM CRKCSTGCPR GMVKVKDCTP WSDIECVHKE SGNGHNIEGR MDPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK |
| Biological Activity: | The ED50, as measured by its ability to inhibit Trail-mediated cytotoxicity in mouse L-929 cells, is less than 1.25ng/ml. |
Human TNFRSF10A is a type 1 transmembrane protein in the TNF R superfamily. TNFRSF10A is not expressed in rodents. The trimeric ligand Trail binds TNFRSF10A and induces apoptosis, and recombinant, soluble forms of the receptor inhibit Trail-induced apoptosis. TNFRSF10A is expressed generally in damaged, infected, and malignant cells. TNFRSF10A functions in immune surveillance, inducing apoptosis in cancer cells but not normal cells.
TRAIL R1 Fc Human Recombinant (24-240) produced in E.Coli is a non-glycosylated, disulfide-linked homodimer containing two polypeptide chains of 217 amino acids each and having a molecular mass of 41kDa [including tag] each chain. TRAILR1 is fused to a 236 amino acid Fc tag at C-terminal and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TRAIL-R1: Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF- kappa-B. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 8p21 Cellular Component: plasma membrane; integral to membrane Molecular Function:protein binding; protease binding; death receptor activity; TRAIL binding; receptor activity; transcription factor binding Biological Process: caspase activation; induction of apoptosis via death domain receptors; apoptosis; activation of NF-kappaB-inducing kinase; signal transduction |
| NCBI Summary: | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O00220 |
| NCBI GenInfo Identifier: | 313104273 |
| NCBI Gene ID: | 8797 |
| NCBI Accession: | O00220.3 |
| UniProt Secondary Accession: | O00220,Q53Y72, Q96E62, A8K5I4, |
| UniProt Related Accession: | O00220 |
| Molecular Weight: | |
| NCBI Full Name: | Tumor necrosis factor receptor superfamily member 10A |
| NCBI Synonym Full Names: | tumor necrosis factor receptor superfamily, member 10a |
| NCBI Official Symbol: | TNFRSF10A�� |
| NCBI Official Synonym Symbols: | DR4; APO2; CD261; TRAILR1; TRAILR-1�� |
| NCBI Protein Information: | tumor necrosis factor receptor superfamily member 10A; TRAIL-R1; TRAIL receptor 1; death receptor 4; cytotoxic TRAIL receptor; TNF-related apoptosis-inducing ligand receptor 1; tumor necrosis factor receptor superfamily member 10a variant 2 |
| UniProt Protein Name: | Tumor necrosis factor receptor superfamily member 10A |
| UniProt Synonym Protein Names: | Death receptor 4; TNF-related apoptosis-inducing ligand receptor 1; TRAIL receptor 1; TRAIL-R1; CD_antigen: CD261 |
| UniProt Gene Name: | TNFRSF10A�� |
| UniProt Entry Name: | TR10A_HUMAN |
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