Human TNFR2 Fc Recombinant Protein (RPPB1015)
- SKU:
- RPPB1015
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P20333
- Research Area:
- Cytokines
Description
| Product Name: | Human TNFR2 Fc Recombinant Protein |
| Product Code: | RPPB1015 |
| Size: | 50µg |
| Species: | Human |
| Target: | TNFR2 Fc |
| Synonyms: | Tumor necrosis factor receptor superfamily member 1B,Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, CD120b antigen, Etanercept, TBPII, TNFBR, TNFR80, TNF-R75, p75TNFR, TNF-R-II. |
| Source: | Chinese Hamster Ovary Cells (CHO) |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Each mg contains 1.6mg mannitol, 0.4 mg sucrose and 48 �g tromethamine. |
| Solubility: | It is recommended to reconstitute the lyophilized TNFR2 in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Tumor Necrosis Factor Receptor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNFR2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(c) Analysis by SDS-PAGE. |
| Biological Activity: | Potency is determined by its ability to neutralize TNF-alpha mediated growth inhibition of A375 cells, corresponding to a Specific Activity of 17,000,000 IU/mg. |
TNFR binds specifically to tumor necrosis factor (TNF) and blocks its interaction with cell surface TNF receptors. TNF is a naturally occurring cytokine that is involved in normal inflammatory and immune responses. It plays an important role in the inflammatory processes of rheumatoid arthritis (RA), polyarticular-course juvenile rheumatoid arthritis (JRA), and ankylosing spondylitis and the resulting joint pathology. In addition, TNF plays a role in the inflammatory process of plaque psoriasis. Elevated levels of TNF are found in involved tissues and fluids of patients with RA, psoriatic arthritis, ankylosing spondylitis (AS), and plaque psoriasis. Two distinct receptors for TNF (TNFRs), a 55 kilodalton protein (p55) and a 75 kilodalton protein (p75), exist naturally as monomeric molecules on cell surfaces and in soluble forms. Biological activity of TNF is dependent upon binding to either cell surface TNFR. Recombinant Human TNFR is a dimeric soluble form of the p75 TNF receptor that can bind to two TNF molecules.It inhibits the activity of TNF in vitro and has been shown to affect several animal models of inflammation, including murine collagen-induced arthritis.
Recombinant Human Tumor Necrosis Factor Receptor 2 Fusion Protein produced in CHO is a dimeric, glycosylated, polypeptide chain consisting of the extracellular ligand-binding portion of the human 75 kilo Dalton (p75) tumor necrosis factor receptor 2 (TNFR2) linked to the Fc portion of human IgG1. The Fc component of TNFR2 contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. It consists of 934 amino acids and has an apparent molecular weight of approximately 150 kilo Daltons.The TNFR2 is purified by standard chromatographic techniques.
| UniProt Protein Function: | TNF-R2: Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. Binds to TRAF2. Interacts with BMX. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Receptor, cytokine Chromosomal Location of Human Ortholog: 1p36.22 Cellular Component: cell soma; perinuclear region of cytoplasm; integral to membrane; plasma membrane; extracellular region; nucleus; lipid raft Molecular Function:protein binding; tumor necrosis factor receptor activity; ubiquitin protein ligase binding Biological Process: tumor necrosis factor-mediated signaling pathway; DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of inflammatory response; immune response; RNA destabilization; inflammatory response; positive regulation of membrane protein ectodomain proteolysis; aging |
| NCBI Summary: | The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P20333 |
| NCBI GenInfo Identifier: | 21264534 |
| NCBI Gene ID: | 7133 |
| NCBI Accession: | P20333.3 |
| UniProt Secondary Accession: | P20333,Q16042, Q6YI29, Q9UIH1, B1AJZ3, |
| UniProt Related Accession: | P20333 |
| Molecular Weight: | 28,461 Da |
| NCBI Full Name: | Tumor necrosis factor receptor superfamily member 1B |
| NCBI Synonym Full Names: | tumor necrosis factor receptor superfamily, member 1B |
| NCBI Official Symbol: | TNFRSF1B�� |
| NCBI Official Synonym Symbols: | p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II�� |
| NCBI Protein Information: | tumor necrosis factor receptor superfamily member 1B; TNF-R2; TNF-RII; p75 TNF receptor; p80 TNF-alpha receptor; soluble TNFR1B variant 1; tumor necrosis factor receptor 2; tumor necrosis factor beta receptor; tumor necrosis factor receptor type II; tumor necrosis factor binding protein 2 |
| UniProt Protein Name: | Tumor necrosis factor receptor superfamily member 1B |
| UniProt Synonym Protein Names: | Tumor necrosis factor receptor 2; TNF-R2; Tumor necrosis factor receptor type II; TNF-RII; TNFR-II; p75; p80 TNF-alpha receptor; CD_antigen: CD120b; INN: EtanerceptCleaved into the following 2 chains:Tumor necrosis factor receptor superfamily member 1b, membrane form; Tumor necrosis factor-binding protein 2Alternative name(s):TBP-2; TBPII |
| UniProt Gene Name: | TNFRSF1B�� |
| UniProt Entry Name: | TNR1B_HUMAN |
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