Human TNF Receptor Associated Factor 6 (TRAF6) ELISA Kit
The Human TNF Receptor-Associated Factor 6 (TRAF6) ELISA Kit is specially designed for the accurate measurement of TRAF6 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.TRAF6 is a key protein involved in various signaling pathways, particularly in the regulation of immune responses and inflammation. Dysregulation of TRAF6 has been associated with autoimmune diseases, inflammatory disorders, and certain types of cancer, highlighting its importance as a potential therapeutic target.
By providing a reliable tool for the quantification of TRAF6 levels, this ELISA kit enables researchers to better understand the role of TRAF6 in disease pathology and develop novel treatment strategies. With its ease of use and accuracy, the Human TRAF6 ELISA Kit is an indispensable resource for studies aimed at unraveling the molecular mechanisms underlying human health and disease.
Product Name:
Human TNF Receptor Associated Factor 6 (TRAF6) ELISA Kit
Product Code:
HUDL02984
Size:
96 Assays
Target:
TRAF6
Synonyms:
RNF85, E3 ubiquitin-protein ligase TRAF6, Interleukin-1 signal transducer
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
