The Human TLR4 (Toll-Like Receptor 4) ELISA Kit is meticulously crafted for the precise quantitative measurement of Toll-Like Receptor 4 in diverse human biological samples. Toll-Like Receptor 4 plays a crucial role in the innate immune response, recognizing pathogen-associated molecular patterns and activating the body's defense mechanisms. Its involvement in inflammation and immune signaling pathways makes it a key target for understanding immune responses and inflammatory diseases. Our ELISA kit offers outstanding sensitivity and specificity, ensuring accurate and reproducible results in your research endeavors.
Manufactured with rigorous quality control measures, this kit delivers reliable performance and user-friendly protocols, making it an excellent tool for investigating the functions and implications of Toll-Like Receptor 4 in immune responses. Trust Assay Genie's TLR4 ELISA Kit for dependable quantification and in-depth exploration of this essential biomarker in your studies.
Product Name:
Human TLR4 (Toll-Like Receptor 4) ELISA Kit
SKU:
AEES00152
Target:
Human TLR4 (Toll-Like Receptor 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TLR4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TLR4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TLR4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TLR4. You can calculate the concentration of Human TLR4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-107
99-109
101-112
Average (%)
103
103
104
1:4
Range (%)
94-106
86-95
93-105
Average (%)
99
92
97
1:8
Range (%)
98-108
102-113
91-105
Average (%)
101
105
97
1:16
Range (%)
97-103
90-98
89-100
Average (%)
100
94
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-110
105
EDTA plasma (n=5)
91-105
99
Cell culture media (n=5)
92-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.09
231.76
832.13
93.11
217.41
859.18
Standard deviation
5.17
10.52
36.11
4.96
10.39
42.01
C V (%)
5.55
4.54
4.34
5.33
4.78
4.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TLR4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TLR4 in samples. No significant cross-reactivity or interference between Human TLR4 and analogues was observed.
