The Human TLR3 Polyclonal Antibody is a valuable tool for researchers studying Toll-like receptor 3 (TLR3), a key player in the innate immune response to viral infections. This antibody, produced in rabbits, is highly specific to human TLR3 and is validated for use in various applications, including Western blotting and immunohistochemistry.TLR3 is known for recognizing double-stranded RNA and initiating a signaling cascade that leads to the production of pro-inflammatory cytokines and type I interferons. This antibody binds specifically to TLR3, allowing researchers to detect and quantify TLR3 expression in different cell types and tissues.
Understanding the role of TLR3 in the immune response is essential for developing new therapies and vaccines targeting viral infections. This antibody is a valuable tool for researchers investigating TLR3 function in diseases such as respiratory infections, autoimmune disorders, and cancer.Overall, the Human TLR3 Polyclonal Antibody is a reliable reagent for studying the role of TLR3 in the immune response and its potential as a therapeutic target in various diseases.
Product Name:
Human TLR3 Polyclonal Antibody
SKU:
CAB24885
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant Protein corresponding to a sequence within amino acids 24-339 of human Human TLR3(NP_003256.1).
TLR3; CD283; IIAE2; toll-like receptor 3; Human TLR3
Positive Sample:
293T transfected with TLR3
Conjugate:
Unconjugated
Cellular Localization:
Early endosome, Endoplasmic reticulum membrane, Endosome membrane, Single-pass type I membrane protein.
Calculated MW:
72kDa/103kDa
Observed MW:
130kDa
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene.
Purification Method:
Affinity purification
Gene ID:
7098
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with TLR3 using Human TLR3 Rabbit pAb(CAB24885) at 1:800 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 10s.
