The Human TLN (Talin) ELISA Kit is meticulously designed to quantitatively detect and measure levels of Talin in various human biological samples. Talin, a vital cytoskeletal protein, plays a crucial role in cell adhesion and interactions with the extracellular matrix, serving as a key regulator of cellular processes like migration, growth, and differentiation. Accurate measurement of Talin with our ELISA kit provides valuable insights into cell adhesion dynamics, cytoskeletal organization, and cellular signaling pathways.
This kit ensures exceptional sensitivity and specificity, guaranteeing reliable and reproducible results. Engineered under strict quality control measures, it delivers robust performance and user-friendly operation, making it an optimal choice for both research and clinical applications. Rely on the Human TLN ELISA Kit for precise and dependable quantification of this essential biomarker in your studies.
Product Name:
Human TLN (Talin) ELISA Kit
SKU:
AEES00108
Target:
Human TLN (Talin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TLN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TLN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TLN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TLN. You can calculate the concentration of Human TLN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-97
90-103
93-107
Average (%)
91
97
100
1:4
Range (%)
84-94
96-105
89-102
Average (%)
90
99
95
1:8
Range (%)
96-110
100-112
100-109
Average (%)
103
104
104
1:16
Range (%)
90-99
85-94
96-107
Average (%)
94
91
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-102
96
EDTA plasma (n=5)
97-111
103
Cell culture media (n=5)
97-107
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.93
22.21
93.02
9.67
24.37
84.69
Standard deviation
0.5
1.05
4.43
0.56
1.11
3.41
C V (%)
5.04
4.73
4.76
5.79
4.55
4.03
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TLN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TLN in samples. No significant cross-reactivity or interference between Human TLN and analogues was observed.
