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Human TKT Recombinant Protein (RPPB2329)

SKU:
RPPB2329
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P29401
Research Area:
Enzymes
€309
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Description

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Product Name:Human TKT Recombinant Protein
Product Code:RPPB2329
Size:10µg
Species:Human
Target:TKT
Synonyms:Transketolase, TK, TKT1, EC 2.2.1.1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The TKT solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM Nacl, 1mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MESYHKPDQQ KLQALKDTAN RLRISSIQAT TAAGSGHPTS CCSAAEIMAV LFFHTMRYKS QDPRNPHNDR FVLSKGHAAP ILYAVWAEAG FLAEAELLNL RKISSDLDGH PVPKQAFTDV ATGSLGQGLG AACGMAYTGK YFDKASYRVY CLLGDGELSE GSVWEAMAFA SIYKLDNLVA ILDINRLGQS DPAPLQHQMD IYQKRCEAFG WHAIIVDGHS VEELCKAFGQ AKHQPTAIIA KTFKGRGITG VEDKESWHGK PLPKNMAEQI IQEIYSQIQS KKKILATPPQ EDAPSVDIAN IRMPSLPSYK VGDKIATRKA YGQALAKLGH ASDRIIALDG DTKNSTFSEI FKKEHPDRFI ECYIAEQNMV SIAVGCATRN RTVPFCSTFA AFFTRAFDQI RMAAISESNI NLCGSHCGVS IGEDGPSQMA LEDLAMFRSV PTSTVFYPSD GVATEKAVEL AANTKGICFI RTSRPENAII YNNNEDFQVG QAKVVLKSKD DQVTVIGAGV TLHEALAAAE LLKKEKINIR VLDPFTIKPL DRKLILDSAR ATKGRILTVE DHYYEGGIGE AVSSAVVGEP GITVTHLAVN RVPRSGKPAE LLKMFGIDRD AIAQAVRGLI TKA

TKT is a thiamine-dependent enzyme that takes part in the channeling of leftover sugar phosphates to glycolysis in the pentose phosphate pathway. Multiple alternatively spliced variants are known that encode the same protein.

TKT Human Recombinant produced in�E. coli is a single polypeptide chain containing 643 amino acids (1-623) and having a molecular mass of 70.0kDa.TKT is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:TKT: Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate. Homodimer. Belongs to the transketolase family.
UniProt Protein Details:

Protein type:Transferase; EC 2.2.1.1; Carbohydrate Metabolism - pentose phosphate pathway

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: nucleoplasm; peroxisome; nucleus; cytosol; vesicle

Molecular Function:transketolase activity; protein homodimerization activity; metal ion binding; cofactor binding

Biological Process: pentose-phosphate shunt; regulation of growth; carbohydrate metabolic process; energy reserve metabolic process; glyceraldehyde-3-phosphate biosynthetic process; pentose-phosphate shunt, non-oxidative branch; xylulose biosynthetic process; pathogenesis

Disease: Wernicke-korsakoff Syndrome

NCBI Summary:This gene encodes a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012]
UniProt Code:P29401
NCBI GenInfo Identifier:1729976
NCBI Gene ID:7086
NCBI Accession:P29401.3
UniProt Secondary Accession:P29401,Q8TBA3, Q96HH3, A8K089, B4DE31, E7EPA7,
UniProt Related Accession:P29401
Molecular Weight:623
NCBI Full Name:Transketolase
NCBI Synonym Full Names:transketolase
NCBI Official Symbol:TKT��
NCBI Official Synonym Symbols:TK; TKT1; HEL107��
NCBI Protein Information:transketolase; epididymis luminal protein 107
UniProt Protein Name:Transketolase
Protein Family:Transketolase
UniProt Gene Name:TKT��
UniProt Entry Name:TKT_HUMAN

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