Human TK2 Recombinant Protein (RPPB2668)
- SKU:
- RPPB2668
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O00142
- Research Area:
- Enzymes
Description
| Product Name: | Human TK2 Recombinant Protein |
| Product Code: | RPPB2668 |
| Size: | 10µg |
| Species: | Human |
| Target: | TK2 |
| Synonyms: | Thymidine kinase 2 mitochondrial, Mt-TK, TK2, MTTK, MTDPS2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | TK2 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer, pH8.0, 30% glycerol, 2mM DTT and 200mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMVQRRA WPPDKEQEKE KKSVICVEGN IASGKTTCLE FFSNATDVEV LTEPVSKWRN VRGHNPLGLM YHDASRWGLT LQTYVQLTML DRHTRPQVSS VRLMERSIHS ARYIFVENLY RSGKMPEVDY VVLSEWFDWI LRNMDVSVDL IVYLRTNPET CYQRLKKRCR EEEKVIPLEY LEAIHHLHEE WLIKGSLFPM AAPVLVIEAD HHMERMLELF EQNRDRILTP ENRKHCP |
Thymidine kinase 2 mitochondrial (TK2) is a member of the DCK/DGK family. TK2 is an enzyme, a phosphotransferase (a kinase): 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase. Thymidine kinase is found in most living cells. Thymidine kinase is present in 2 forms in mammalian cells, TK1 and TK2. Thymidine kinases have a central function in the synthesis of DNA and thus in cell division, since they are part of the exceptional reaction chain to introduce deoxythymidine into the DNA. TK2 is a deoxyribonucleoside kinase which specifically phosphorylates thymidine, deoxycytidine, and deoxyuridine. TK2 localizes to the mitochondria and is essential for mitochondrial DNA synthesis. TK2 gene defects are a cause of mitochondrial DNA depletion syndrome type 2 (MTDPS2).
TK2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 257 amino acids (34-265 a.a) and having a molecular mass of 30.2kDa.TK2 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TK2: Deoxyribonucleoside kinase that phosphorylates thymidine, deoxycytidine, and deoxyuridine. Also phosphorylates anti-viral and anti-cancer nucleoside analogs. Defects in TK2 are a cause of mitochondrial DNA depletion syndrome type 2 (MTDPS2). A disorder characterized primarily by childhood onset of muscle weakness associated with depletion of mtDNA in skeletal muscle. There is wide clinical variability; some patients have onset in infancy and show a rapidly progressive course with early death due to respiratory failure, whereas others have later onset of a slowly progressive myopathy. Belongs to the DCK/DGK family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Kinase, other; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 2.7.1.21; Nucleotide Metabolism - pyrimidine; Mitochondrial Chromosomal Location of Human Ortholog: 16q22-q23.1 Cellular Component: mitochondrial matrix Molecular Function:nucleoside kinase activity Biological Process: nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; pyrimidine nucleoside salvage Disease: Mitochondrial Dna Depletion Syndrome 2 (myopathic Type) |
| NCBI Summary: | This gene encodes a deoxyribonucleoside kinase that specifically phosphorylates thymidine, deoxycytidine, and deoxyuridine. The encoded enzyme localizes to the mitochondria and is required for mitochondrial DNA synthesis. Mutations in this gene are associated with a myopathic form of mitochondrial DNA depletion syndrome. Alternate splicing results in multiple transcript variants encoding distinct isoforms, some of which lack transit peptide, so are not localized to mitochondria. [provided by RefSeq, Dec 2012] |
| UniProt Code: | O00142 |
| NCBI GenInfo Identifier: | 254763443 |
| NCBI Gene ID: | 7084 |
| NCBI Accession: | O00142.4 |
| UniProt Secondary Accession: | O00142,O15238, B4DGJ7, B4DZK7, B7ZAB1, E9PH08, |
| UniProt Related Accession: | O00142 |
| Molecular Weight: | 20,281 Da |
| NCBI Full Name: | Thymidine kinase 2, mitochondrial |
| NCBI Synonym Full Names: | thymidine kinase 2, mitochondrial |
| NCBI Official Symbol: | TK2�� |
| NCBI Official Synonym Symbols: | MTTK; SCA31; MTDPS2�� |
| NCBI Protein Information: | thymidine kinase 2; thymidine kinase 2, mitochondrial |
| UniProt Protein Name: | Thymidine kinase 2, mitochondrial |
| UniProt Synonym Protein Names: | Mt-TK |
| Protein Family: | Thymidine kinase |
| UniProt Gene Name: | TK2�� |
| UniProt Entry Name: | KITM_HUMAN |
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