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Human TK1 Recombinant Protein (RPPB2667)

SKU:
RPPB2667
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P04183
Research Area:
Enzymes
€309
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Description

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Product Name:Human TK1 Recombinant Protein
Product Code:RPPB2667
Size:20µg
Species:Human
Target:TK1
Synonyms:Thymidine kinase 1 soluble, thymidine kinase cytosolic, TK2, EC 2.7.1.21.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The TK1 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMSCINL PTVLPGSPSK TRGQIQVILG PMFSGKSTEL MRRVRRFQIA QYKCLVIKYA KDTRYSSSFC THDRNTMEAL PACLLRDVAQ EALGVAVIGI DEGQFFPDIV EFCEAMANAG KTVIVAALDG TFQRKPFGAI LNLVPLAESV VKLTAVCMEC FREAAYTKRL GTEKEVEVIG GADKYHSVCR LCYFKKASGQ PAGPDNKENC PVPGKPGEAV AARKLFAPQQ ILQCSPAN

Thymidine Kinase 1 (TK1) is a phosphotransferase (a kinase): 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase. TK1 is present in 2 forms in mammalian cells, TK1 and TK2. Thymidine kinases hold a main function in the synthesis of DNA and thus in cell division, as they are part of the distinctive reaction chain to introduce deoxythymidine (present in the body fluids as a result of degradation of DNA from food and from dead cells) into the DNA. Thymidine kinase is necessary for the action of many antiviral drugs. Thymidine kinase is used to select hybridoma cell lines in production of monoclonal antibodies.

TK1 Human Recombinant produced in E. coli is a single polypeptide chain containing 258 amino acids (1-234) and having a molecular mass of 28.0 kDa.TK1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:TK: cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.
UniProt Protein Details:

Protein type:EC 2.7.1.21; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - pyrimidine; Kinase, other

Chromosomal Location of Human Ortholog: 17q23.2-q25.3

Cellular Component: cytosol

Molecular Function:identical protein binding; nucleoside kinase activity; zinc ion binding; thymidine kinase activity; ATP binding

Biological Process: response to nutrient levels; skeletal muscle cell proliferation; nucleobase, nucleoside and nucleotide metabolic process; response to toxin; liver development; protein homotetramerization; fetal process involved in parturition; deoxyribonucleoside monophosphate biosynthetic process; response to cortisol stimulus; pyrimidine base metabolic process; gut development; response to copper ion; nucleotide biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; thymidine metabolic process; pyrimidine nucleoside salvage; phosphorylation

UniProt Code:P04183
NCBI GenInfo Identifier:23503074
NCBI Gene ID:7083
NCBI Accession:P04183.2
UniProt Secondary Accession:P04183,Q969V0, Q9UMG9, B2RC58,
UniProt Related Accession:P04183
Molecular Weight:234
NCBI Full Name:Thymidine kinase, cytosolic
NCBI Synonym Full Names:thymidine kinase 1, soluble
NCBI Official Symbol:TK1��
NCBI Official Synonym Symbols:TK2��
NCBI Protein Information:thymidine kinase, cytosolic; thymidine kinase-1; thymidine kinase 1 soluble isoform
UniProt Protein Name:Thymidine kinase, cytosolic
Protein Family:Thymidine kinase
UniProt Gene Name:TK1��
UniProt Entry Name:KITH_HUMAN

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