Human TIPIN Recombinant Protein (RPPB4965)
- SKU:
- RPPB4965
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9BVW5
Description
| Product Name: | Human TIPIN Recombinant Protein |
| Product Code: | RPPB4965 |
| Size: | 10µg |
| Species: | Human |
| Target: | TIPIN |
| Synonyms: | TIMELESS Interacting Protein, CSM3 Homolog. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The TIPIN solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.15M NaCl, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMLEPQEN GVIDLPDYEH VEDETFPPFP PPASPERQDG EGTEPDEESG NGAPVPVPPK RTVKRNIPKL DAQRLISERG LPALRHVFDK AKFKGKGHEA EDLKMLIRHM EHWAHRLFPK LQFEDFIDRV EYLGSKKEVQ TCLKRIRLDL PILHEDFVSN NDEVAENNEH DVTSTELDPF LTNLSESEMF ASELSRSLTE EQQQRIERNK QLALERRQAK LLSNSQTLGN DMLMNTPRAH TVEEVNTDED QKEESNGLNE DILDNPCNDA IANTLNEEET LLDQSFKNVQ QQLDATSRNI TEAR |
TIPIN is a member of the CSM3 family. TIPIN protein is essential for normal advancement of S-phase and vital for cell existence after DNA damage or replication stress. TIPIN is specifically necessary for the ATR - CHEK1 pathway in the replication checkpoint induced by ultraviolet light.
TIPIN Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 324 amino acids (1-301) and having a molecular mass of 36.9kDa.TIPIN is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TIPIN: Required for normal progression of S-phase. Important for cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. Belongs to the CSM3 family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 15q22.31 Cellular Component: intracellular membrane-bound organelle; nuclear chromatin; nucleoplasm; nucleus Molecular Function:DNA binding; protein binding Biological Process: DNA replication; DNA replication checkpoint; intra-S DNA damage checkpoint; positive regulation of cell proliferation; regulation of DNA replication during S phase; replication fork arrest; replication fork protection |
| NCBI Summary: | The protein encoded by this gene is part of the replisome complex, a group of proteins that support DNA replication. It binds TIM, which is involved in circadian rhythm regulation, and aids in protecting cells against DNA damage and stress. Two pseudogenes and two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014] |
| UniProt Code: | Q9BVW5 |
| NCBI GenInfo Identifier: | 296452932 |
| NCBI Gene ID: | 54962 |
| NCBI Accession: | Q9BVW5.2 |
| UniProt Secondary Accession: | Q9BVW5,Q9NWZ6, B2CW64, |
| UniProt Related Accession: | Q9BVW5 |
| Molecular Weight: | 35kDa |
| NCBI Full Name: | TIMELESS-interacting protein |
| NCBI Synonym Full Names: | TIMELESS interacting protein |
| NCBI Official Symbol: | TIPIN�� |
| NCBI Protein Information: | TIMELESS-interacting protein |
| UniProt Protein Name: | TIMELESS-interacting protein |
| Protein Family: | TIMELESS-interacting protein |
| UniProt Gene Name: | TIPIN�� |
