Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) CLIA Kit
The Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) CLIA Kit is a reliable and accurate tool for the detection of TIMP-3 levels in human tissue samples. With high sensitivity and specificity, this kit provides researchers with precise and reproducible results for a variety of applications.TIMP-3 is a key inhibitor of metalloproteinases, enzymes involved in tissue remodeling and extracellular matrix degradation. Imbalances in TIMP-3 levels have been linked to various diseases such as cancer, cardiovascular disorders, and inflammatory conditions, making it a valuable biomarker for studying disease mechanisms and developing targeted therapies.
With its user-friendly format and robust performance, the Human TIMP-3 CLIA Kit is an essential tool for researchers looking to explore the role of TIMP-3 in health and disease. Get reliable results and advance your research with this cutting-edge kit from Assay Genie.
Product Name:
Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) CLIA Kit
SKU:
HUES00831
Target:
Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TIMP-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TIMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TIMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TIMP-3. You can calculate the concentration of Human TIMP-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
97-113
85-100
Average (%)
99
104
92
1:4
Range (%)
88-103
90-106
84-95
Average (%)
94
97
90
1:8
Range (%)
84-97
89-100
89-102
Average (%)
91
95
95
1:16
Range (%)
94-109
93-108
85-96
Average (%)
101
100
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
91
EDTA plasma (n=5)
87-99
92
Cell culture media (n=5)
97-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.26
118.42
407.87
48.2
110.76
424.16
Standard deviation
4.37
10.5
39.03
5.25
9.0
47.17
C V (%)
8.21
8.87
9.57
10.89
8.13
11.12
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TIMP-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TIMP-3 in samples. No significant cross-reactivity or interference between Human TIMP-3 and analogues was observed.
