Human TIE1 Fc Recombinant Protein (RPPB2665)
- SKU:
- RPPB2665
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P35590
- Research Area:
- Enzymes
Description
| Product Name: | Human TIE1 Fc Recombinant Protein |
| Product Code: | RPPB2665 |
| Size: | 10µg |
| Species: | Human |
| Target: | TIE1 Fc |
| Synonyms: | Tyrosine kinase with immunoglobulin-like and EGF-like domains 1, JTK14, TIE, TIE1. |
| Source: | Insect Cells |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | TIE-1 Fc Chimera was lyophilized from a concentrated (1mg/ml) sterile solution containing 20mM Tris, 0.5M NaCl, 10% Sucrose. |
| Solubility: | It is recommended to reconstitute the lyophilized TIE-1 Fc Chimera in sterile water not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized sTIE-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TIE-1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by:(A) Analysis by RP-HPLC.(B) Analysis by SDS-PAGE. |
TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-1 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Whereas two ligands have been described for TIE-2 [angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2)], so far no ligand was found for TIE-1.
Soluble TIE-1 Human Recombinant fused with the Fc part of human IgG1 produced in baculovirus is a homodimeric, glycosylated, polypeptide containing 749 amino acids and having a total molecular mass of 250 kDa. Human TIE-1/Fc monomer has a calculated molecular mass of approximately 105 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 125 kDa protein in SDS-PAGE under reducing conditions.The TIE1 Fc Chimera is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TIE1: Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily. |
| UniProt Protein Details: | Protein type:Kinase, protein; Protein kinase, TK; Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; Tie family Chromosomal Location of Human Ortholog: 1p34-p33 Cellular Component: integral to plasma membrane Molecular Function:protein binding; transmembrane receptor protein tyrosine kinase activity; ATP binding Biological Process: negative regulation of angiogenesis; peptidyl-tyrosine phosphorylation; response to retinoic acid; in utero embryonic development; mesoderm development; angiogenesis; signal transduction; vasculogenesis; negative regulation of cell migration; plasma membrane fusion |
| NCBI Summary: | This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
| UniProt Code: | P35590 |
| NCBI GenInfo Identifier: | 4885631 |
| NCBI Gene ID: | 7075 |
| NCBI Accession: | NP_005415.1 |
| UniProt Related Accession: | P35590 |
| Molecular Weight: | 125,090 Da |
| NCBI Full Name: | tyrosine-protein kinase receptor Tie-1 isoform 1 |
| NCBI Synonym Full Names: | tyrosine kinase with immunoglobulin-like and EGF-like domains 1 |
| NCBI Official Symbol: | TIE1�� |
| NCBI Official Synonym Symbols: | TIE; JTK14�� |
| NCBI Protein Information: | tyrosine-protein kinase receptor Tie-1; tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 |
| UniProt Protein Name: | Tyrosine-protein kinase receptor Tie-1 |
| UniProt Gene Name: | TIE1�� |
| UniProt Entry Name: | TIE1_HUMAN |
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