Human Thyroid Hormone Receptor Interactor 6 (TRIP6) ELISA Kit
The Human Thyroid Hormone Receptor Interactor 6 (TRIP6) ELISA Kit is a powerful tool for detecting levels of TRIP6 in human samples. TRIP6 is a key protein that interacts with thyroid hormone receptors, playing a critical role in various biological processes.This ELISA kit offers high sensitivity and specificity, ensuring accurate and reproducible results in serum, plasma, and cell culture supernatants. It is ideal for researchers studying the role of TRIP6 in cellular function, growth, and development.
TRIP6 is known to be involved in cell adhesion, migration, and signaling pathways, making it a valuable target for understanding diseases such as cancer, cardiovascular disorders, and neurological conditions. By measuring TRIP6 levels, researchers can gain insights into disease mechanisms and potential therapeutic strategies. Overall, the Human TRIP6 ELISA Kit from Assay Genie is a reliable tool for studying the role of this important protein in various biological processes and disease states.
Product Name:
Human Thyroid Hormone Receptor Interactor 6 (TRIP6) ELISA Kit
Product Code:
HUDL02992
Size:
96 Assays
Target:
TRIP6
Synonyms:
OIP1, ZRP-1, Opa-interacting protein 1, Zyxin-related protein 1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
