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Human Thymus Activation Regulated Chemokine (TARC) ELISA Kit

SKU:
HUDL02830
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q92583
Sensitivity:
10.5pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
CCL17,ABCD-2,SCYA17,TARC,Small Inducible Cytokine Subfamily A(Cys-Cys)Member 17,Small-inducible cytokine A17
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

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Human Thymus Activation Regulated Chemokine (TARC) ELISA Kit

The Human Thymus Activation Regulated Chemokine (TARC) ELISA Kit is specifically designed for the accurate quantification of TARC levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring trustworthy and consistent results for various research purposes.TARC is an important chemokine that plays a key role in regulating immune responses and inflammation. Abnormal levels of TARC have been associated with various inflammatory and autoimmune disorders, making it a valuable biomarker for studying these conditions and developing potential therapies.

With its reliable performance and wide range of applications, the Human TARC ELISA Kit is an essential tool for researchers working in the fields of immunology, inflammation, and autoimmune diseases. Get accurate and precise results with this advanced ELISA kit from Assay Genie.

Product Name:Human Thymus Activation Regulated Chemokine (TARC) ELISA Kit
Product Code:HUDL02830
Size:96 Assays
Target:TARC
Synonyms:CCL17, ABCD-2, SCYA17, TARC, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 17, Small-inducible cytokine A17
Detection Method:Sandwich
Range:31.25-2,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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