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Human Thymine DNA Glycosylase (TDG) ELISA Kit

SKU:
HUDL02848
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q13569
Sensitivity:
0.038ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
G/T Mismatch-Specific Thymine DNA Glycosylase,Thymine-DNA glycosylase
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Thymine DNA Glycosylase (TDG) ELISA Kit

The Human Thymine DNA glycosylase (TDG) ELISA Kit is specifically designed for the precise detection of TDG levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for various research purposes.TDG plays a critical role in DNA repair by removing inappropriate bases paired with guanine in DNA. Dysregulation of TDG has been implicated in various diseases, including cancer and neurological disorders, making it a valuable biomarker for understanding disease mechanisms and developing targeted treatments.

With its advanced technology and reliable performance, the Human TDG ELISA Kit is an invaluable tool for researchers seeking to explore the role of TDG in health and disease. Order yours today from AssayGenie and take your research to the next level.

Product Name:Human Thymine DNA Glycosylase (TDG) ELISA Kit
Product Code:HUDL02848
Size:96 Assays
Target:TDG
Synonyms:G/T Mismatch-Specific Thymine DNA Glycosylase, Thymine-DNA glycosylase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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